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![Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Ythdc1-cKO mouse ES cells were treated by 4OHT. |Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC66394-01_WB_01_83-136.jpg)
