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Affinity purification

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
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Western blot analysis of various lysates using HB-EGF Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

HB-EGF Rabbit Recombinant Antibody (81-203)

CATALOG NUMBER: 81-203

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from K-562 cells, using HBG1 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

HBG1/2 Rabbit Recombinant Antibody (81-065)

CATALOG NUMBER: 81-065

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using HBG1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): Ramos, HeLa.|Exposure time: 10 s.

HBG1/2 Rabbit Recombinant Antibody (81-066)

CATALOG NUMBER: 81-066

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using hCG Beta Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): THP-1|Exposure time: 45s.

hCG Beta Rabbit Recombinant Antibody (82-881)

CATALOG NUMBER: 82-881

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from BeWo cells using hCG beta Rabbit mAb at 5000 dilution incubated overnight at 4℃. BeWo cells were treated with PNGase (6 U/μL) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20 s.

hCG beta Rabbit Recombinant Antibody (83-616)

CATALOG NUMBER: 83-616

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using HCoV-229E Spike S1 Rabbit mAb at 1:800 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Negative control (NC): 293T.|Exposure time: 180s.

HCoV-229E Spike S1 Rabbit Recombinant Antibody (82-305)

CATALOG NUMBER: 82-305

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
HCoV-229E
List Size:
100 uL
Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag) using HCoV-229E Spike S2 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 100ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

HCoV-229E Spike S2 Rabbit Recombinant Antibody (82-304)

CATALOG NUMBER: 82-304

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
HCoV-229E
List Size:
100 uL
Western blot analysis of recombinant Human coronavirus (HCoV-NL63) Spike Protein (S1+S2 ECD His Tag) using HCoV-NL63 Spike S2 Rabbit mAb at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

HCoV-NL63 Spike S2 Rabbit Recombinant Antibody (82-323)

CATALOG NUMBER: 82-323

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Humancoronavirus(HCoV-NL63), WideRange
List Size:
100 uL
Western blot analysis of various lysates, using HCoV-OC43 Spike S1 Rabbit mAb at 1:8000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

HCoV-OC43 Spike S1 Rabbit Recombinant Antibody (82-339)

CATALOG NUMBER: 82-339

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using HCoV-OC43 Spike S1 Rabbit mAb at 1:8000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

HCoV-OC43 Spike S1 Rabbit Recombinant Antibody (82-374)

CATALOG NUMBER: 82-374

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL