Affinity purification

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Product Badges
Western blot analysis of various lysates using HRAS Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using HRH3 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Rabbit IgG using HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb at 1:1000-1:20000 dilution incubated overnight at 4℃.|Lysates/proteins: 100ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.
Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, WB
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG2 (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG2 (Fc) has highly specific recognition of Human IgG2、Human IgG while no or minimal cross reactivity to Human IgG1、Human IgG4、Human IgM、Rat IgG.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Monkey IgA mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgA mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgA mAb has highly specific recognition of Monkey IgA while no or minimal cross reactivity to Monkey IgD、Monkey IgE、Monkey IgG3、Human IgA、Mouse IgA.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Cynomolgus Monkey
List Size:
100 uL
Dose response curve of HRP conjugated Monkey IgD mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgD mAb in a 3 fold serial dilution from 1μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Monkey IgD mAb has highly specific recognition of Monkey IgD while no or minimal cross reactivity to Monkey IgA、Monkey IgE、Monkey IgG3、Human IgD、Mouse IgD.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Cynomolgus Monkey
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgD (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgD (Fc) has highly specific recognition of Mouse IgD while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2b、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgA、Rat IgG、Human IgG.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgE mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgE mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Mouse IgE mAb has highly specific recognition of Mouse IgE while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2b、Mouse IgG3、Mouse IgM、Mouse IgA、Human IgG、Rat IgG.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Dose response curve of HRP conjugated Mouse IgG1 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG1 mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Mouse IgG1 mAb has highly specific recognition of Mouse IgG1 while no or minimal cross reactivity to Mouse IgG 2a、Mouse IgG 2b、Mouse IgG3、Mouse IgM、Human IgG1、Rat IgG1.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Mouse
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG2b (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1*10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Mouse IgG2b (Fc) has highly specific recognition of Mouse IgG2b while no or minimal cross reactivity to Mouse IgG1、Mouse IgG2a、Mouse IgG2c、Mouse IgG3、Mouse IgM、Mouse IgE、Mouse IgD、Rat IgG、Human IgG.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Mouse
List Size:
100 uL