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Affinity purification

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Species Reactivity
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Dose response curve of HRP conjugated Rabbit Anti-Human IgG4 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG4 mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgG4 while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgM、Human IgE、Human IgD.

HRP-conjugated Rabbit anti-Human IgG4 Recombinant Antibody (84-785)

CATALOG NUMBER: 84-785

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Monkey IgE mAb measured by ELISA. 25 ng of Monkey IgE recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Monkey IgE mAb in a 2 fold serial dilution from 1 μg/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 6.54×10^-2 μg/mL) were calculated using 4 parameter logistic regression.

HRP-conjugated Rabbit anti-Monkey IgE (Fc) Recombinant Antibody (84-784)

CATALOG NUMBER: 84-784

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Monkey
List Size:
100 uL
Western blot analysis of lysates from HeLa, using HRP-conjugated β-Actin Rabbit mAb at 1:2000- 1:640000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10 s.

HRP-conjugated β-Actin Mouse Recombinant Antibody (84-503)

CATALOG NUMBER: 84-503

Clonality:
Monoclonal
Host Species:
Mouse
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using HRP-conjugated β-Actin Rabbit mAb at 1:200000 dilution incubated overnight at 4℃.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10 s.

HRP-conjugated β-Actin Rabbit Recombinant Antibody (84-497)

CATALOG NUMBER: 84-497

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Chicken, Human, Mouse, Pig, Rat, Zebrafish
List Size:
100 uL
Western blot analysis of various lysates using HRPT2 / CDC73 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

HRPT2 / CDC73 Rabbit Recombinant Antibody (82-690)

CATALOG NUMBER: 82-690

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Hsc70-interacting protein (HIP) Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Hsc70-interacting protein (HIP) Rabbit Recombinant Antibody (84-424)

CATALOG NUMBER: 84-424

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Hsc70/HSPA8 Rabbit mAb at 1：1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Hsc70/HSPA8 Rabbit Recombinant Antibody (81-026)

CATALOG NUMBER: 81-026

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using HSD17B13 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

HSD17B13 Rabbit Recombinant Antibody (81-862)

CATALOG NUMBER: 81-862

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using HSD17B2 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): 293F|Exposure time: 30s.

HSD17B2 Rabbit Recombinant Antibody (83-507)

CATALOG NUMBER: 83-507

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using HSD3B1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

HSD3B1 Rabbit Recombinant Antibody (81-443)

CATALOG NUMBER: 81-443

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL