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![Western blot analysis of lysates from U-251 MG cells using [KD Validated] CD90/Thy1 Rabbit mAb at 1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC55647-01_WB_01_82-520.jpg)
![Western blot analysis of various lysates, using [KD Validated] GALC Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC58988-01_WB_01_82-524.jpg)
![Western blot analysis of lysates from wild type(WT) and LAMP1 knockout (KO) HeLa(KO) cells, using [KO Validated] LAMP1/CD107a Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52155-01_KO-WB_01_82-213.jpg)
![Western blot analysis of lysates from wild type (WT) and βIII-Tubulin knockout (KO) HeLa cells using [KO Validated] βIII-Tubulin Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC63789-01_WB_01_82-857.jpg)
