Cell Type Marker

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of various lysates, using CD45 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC):MCF7 |Exposure time: 1s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, mIHC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Flow cytometry: 1X10^6 293T cells (negative control,left) and Jurkat cells (right) were surface-stained with CD45 Rabbit mAb or Rabbit IgG isotype control, followed by PSFlo 405-conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD45RA Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CD45RB Rabbit mAb at 1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): HAP1.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, IF-P, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using CD90/Thy1  Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from PC-3 cells using CD97 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CEND1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from SH-SY5Y cells, using CHAT Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse,Rat
List Size:
100 uL
Confocal imaging of paraffin-embedded Mouse brain tissue using CHAT Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IF/ICC
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Chromogranin A Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL