Cytokines

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Product Badges
Western blot analysis of various lysates using CD30 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): U-937.|Exposure time: 20 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates using CDC42 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:60s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CMTM6 Rabbit mAb at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using CMTM6 Rabbit mAb at1:5000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of recombinant mouse CXCL1/GRO-alpha Protein using CXCL1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 5ng/1ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using CXCL10/IP-10 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with IFN-γ (100 ng/mL), LPS (1 μg/mL) and Brefeldin A (300 ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 10s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CXCL10/IP-10 Rabbit mAb at 1:10000 dilution incubated overnight at 4℃. A549 cells and HT-29 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A549 cells using CXCL10/IP-10 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. A549 cells were treated with TNF-α (20ng/mL) for 6 hours, Brefeldin A (300ng/mL) for 3 hours and IFN-γ (50ng/mL) for 8 hours of stimulation.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL