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![Western blot analysis of various lysates using [KD Validated] ATF1 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73543-01_WB_01_83-564.jpg)
![Western blot analysis of various lysates using [KD Validated] JNK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2908_WB_01_81-997.jpg)
![Western blot analysis of various lysates, using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC0275R-01_WB_01_81-297.jpg)
![Western blot analysis of lysates from wild type (WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T cells, using [KD Validated] PKA C-alpha (PRKACA) Mouse mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC0275-02-1_KO-WB_01_81-993.jpg)
![Western blot analysis of various lysates, using [KO Validated] CDK5 Rabbit mAb at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC53344-01_WB_01_82-647.jpg)
![Western blot analysis of lysates from Mouse brain using [KO Validated] MEK7 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0712_WB_03_81-251.jpg)
![Western blot analysis of lysates from wild type (WT) and N-terminal alpha-synuclein knockout (KO) HIPP Neurons cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] at 1:5000 dilution .|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC5166-01_WB_01_83-469.jpg)
