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![Western blot analysis of lysates from wild type (WT) and Insulin-degrading enzyme/IDE knockdown (KD) 293T cells using [KD Validated] Insulin-degrading enzyme/IDE Rabbit mAb at 1:6000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC74108-01_WB_01_83-610.jpg)
![Western blot analysis of various lysates using [KD Validated] Peroxiredoxin 6 Rabbit mAb at 1:12000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC71035-01_WB_01_83-503.jpg)
![Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC58148-01_WB_01_82-627.jpg)
![Western blot analysis of lysates from wild type (WT) and FTO knockout (KO) 293T(KO) cells, using [KO Validated] FTO Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52115-01_KO-WB_01_82-277.jpg)
