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Immunology Inflammation

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from THP-1 cells using Cleaved Caspase-1 p20 Rabbit mAb at 1:2000 dilution. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Cleaved Caspase-1 p20 Rabbit Recombinant Antibody (82-600)

CATALOG NUMBER: 82-600

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:60s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Cleaved IL-18 (Tyr37) Rabbit Recombinant Antibody (82-854)

CATALOG NUMBER: 82-854

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from Jurkat cells using Cleaved PARP Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Jurkat cells were treated with Etoposide (25 μM) at 37℃ for 5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 0.5s.

Cleaved PARP (Asp214) Rabbit Recombinant Antibody (83-474)

CATALOG NUMBER: 83-474

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and Cleaved PARP1 p25 knockout (KO) cells using Cleaved PARP1 p25 Rabbit mAb at 1:10000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and PARP1 knockout (KO) HeLa cells were treated with Staurosporine(1 μM) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

Cleaved PARP1 p25 Rabbit Recombinant Antibody (81-527)

CATALOG NUMBER: 81-527

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Clusterin Rabbit mAb at 1:1000 dilution.| Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.| Lysates / proteins: 25 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.

Clusterin Rabbit Recombinant Antibody (81-875)

CATALOG NUMBER: 81-875

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using CMTM6 Rabbit mAb at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

CMTM6 Rabbit Recombinant Antibody (81-985)

CATALOG NUMBER: 81-985

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using CMTM6 Rabbit mAb at1:5000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

CMTM6 Rabbit Recombinant Antibody (82-153)

CATALOG NUMBER: 82-153

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL

CMTM6 Rabbit Recombinant Antibody (82-154)

CATALOG NUMBER: 82-154

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using Coagulation factor III/Tissue Factor Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): HEL|Exposure time: 20s.

Coagulation factor III/Tissue Factor Rabbit Recombinant Antibody (82-537)

CATALOG NUMBER: 82-537

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL