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Jak-Stat-IL-6 Receptor Signaling Pathway

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of various lysates, using MEK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

MEK1 Rabbit Recombinant Antibody (82-263)

CATALOG NUMBER: 82-263

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from RD cells, using MEK5 Rabbit mAb at 1：1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

MEK5 Rabbit Recombinant Antibody (81-242)

CATALOG NUMBER: 81-242

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Mras Rabbit mAb at 1:500 dilution incubated overnight at 4°C.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.

Mras Rabbit Recombinant Antibody (81-829)

CATALOG NUMBER: 81-829

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunoprecipitation of NF κB P105 from 300 µg extracts of 293T cells was performed using 3 µg of NF κB P105 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using NF κB P105 Rabbit mAb at a dilution of 1 : 2000.

NF κB P105 Rabbit Recombinant Antibody (83-252)

CATALOG NUMBER: 83-252

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates using NF-κB2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.

NF-κB2 Rabbit Recombinant Antibody (81-523)

CATALOG NUMBER: 81-523

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates, using NF-κB2 Rabbit mAb at 1:700 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

NF-κB2 Rabbit Recombinant Antibody (82-172)

CATALOG NUMBER: 82-172

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Jurkat cells, using Phospho-Akt-T308 Rabbit mAb at1:2000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-Akt-T308 Rabbit Recombinant Antibody (84-648)

CATALOG NUMBER: 84-648

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL