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Jak-Stat-IL-6 Receptor Signaling Pathway

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Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Western blot analysis of lysates from BxPC-3 cells, using Phospho-EGFR-Y1068 Rabbit mAb at 1:1000 dilution. BxPC-3 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-EGFR-Y1068 Rabbit Recombinant Antibody (84-570)

CATALOG NUMBER: 84-570

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 cells using Phospho-EGFR-Y1173 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. A-431 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-EGFR-Y1173 Rabbit Recombinant Antibody (84-569)

CATALOG NUMBER: 84-569

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y845 at 1:800 dilution. A-431 cells were treated with EGF (200 ng/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

Phospho-EGFR-Y845 Rabbit Recombinant Antibody (84-703)

CATALOG NUMBER: 84-703

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from A-431 cells using Phospho-EGFR-Y845 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. A-431 cells were treated with EGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20s.

Phospho-EGFR-Y845 Rabbit Recombinant Antibody (84-738)

CATALOG NUMBER: 84-738

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HuH-7 cells using Phospho-JAK2-Y1007/1008 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. HuH-7 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.

Phospho-JAK2-Y1007/1008 Rabbit Recombinant Antibody (84-775)

CATALOG NUMBER: 84-775

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb at 1:1000 dilution. Both HeLa cells and C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1min.

Phospho-MEK1-S298 Rabbit Recombinant Antibody (84-588)

CATALOG NUMBER: 84-588

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 60 s.

Phospho-MEK1-T292 Rabbit Recombinant Antibody (84-589)

CATALOG NUMBER: 84-589

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-mTOR-S2481 at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated with IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-mTOR-S2481 Rabbit Recombinant Antibody (84-557)

CATALOG NUMBER: 84-557

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL