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Microtubules

Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Conjugate
Product Badges
Western blot analysis of lysates from Mouse brain using Phospho-Camk2-T286 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Mouse brain treated with CIP (1U/ul) at 37℃ for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

Phospho-Camk2-T286 Rabbit Recombinant Antibody (84-683)

CATALOG NUMBER: 84-683

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Neuro-2a cells using Phospho-CDK1-T14 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. Neuro-2a cells were treated with Thymidine (2 nM) for 16 hours and Nocodazoleat (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Phospho-CDK1-T14 Rabbit Recombinant Antibody (84-728)

CATALOG NUMBER: 84-728

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from A-431 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb at 1:19000 dilution incubated overnight at 4℃. A-431 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit Recombinant Antibody (84-765)

CATALOG NUMBER: 84-765

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-CDK1-Y15 Rabbit mAb at 1:2000 dilution. HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3 s.

Phospho-CDK1-Y15 Rabbit Recombinant Antibody (84-656)

CATALOG NUMBER: 84-656

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GSK3α-S21+GSK3β-S9 Rabbit mAb at 1:800 dilution. NIH/3T3 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-GSK3α-S21+GSK3β-S9 Rabbit Recombinant Antibody (84-653)

CATALOG NUMBER: 84-653

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-GSK3β-S9 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20s.

Phospho-GSK3β-S9 Rabbit Recombinant Antibody (84-609)

CATALOG NUMBER: 84-609

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by λ-PP mixed solution (1μL) at 30℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20s.

Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit Recombinant Antibody (84-760)

CATALOG NUMBER: 84-760

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.

Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit Recombinant Antibody (84-621)

CATALOG NUMBER: 84-621

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PAK2-S20 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-PAK2-S20 Rabbit Recombinant Antibody (84-714)

CATALOG NUMBER: 84-714

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-PKC alpha-T638 Rabbit Recombinant Antibody (84-603)

CATALOG NUMBER: 84-603

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL