Obesity

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
Product Badges
IL1B Antibody

CATALOG NUMBER: 16-529

Clonality:
Polyclonal
Host Species:
Rabbit
Tested Applications:
IF, IHC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of Recombinant Mouse IL-1 beta Protein , using IL1β Rabbit mAb at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng/5ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using IL1β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (0.5 μg/mL) for 2 hours after serum starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from U-87 MG cells, using IL1β Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using IL1β Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours and Brefeldin A for 3 hours of stimulation. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
IL6 Antibody

CATALOG NUMBER: 13-129

Clonality:
Polyclonal
Host Species:
Rabbit
Tested Applications:
IF, IHC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
IL6 Antibody

CATALOG NUMBER: 13-918

Clonality:
Polyclonal
Host Species:
Rabbit
Tested Applications:
IF, IHC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
IL6 Antibody

CATALOG NUMBER: 15-666

Clonality:
Polyclonal
Host Species:
Rabbit
Tested Applications:
IF, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
IL6 Antibody

CATALOG NUMBER: 56-451

Clonality:
Polyclonal
Host Species:
Rabbit
Tested Applications:
Flow, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
400 ul
Western blot analysis of lysates from THP-1 cells using IL-6 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA(80 nM) at 37℃ for overnight, LPS (1 μg/mL) and BFA (300 ng/mL) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL