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![Western blot analysis of various lysates using [KO Validated] cIAP1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0168_WB_01_81-580.jpg)
![Western blot analysis of lysates from wild type (WT) and Gasdermin E (N terminal) knockout (KO) HeLa cells using [KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and GSDME knockout (KO) HeLa cells were treated with TNF-α (50ng/mL) and CHX (10μg/mL) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.|WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70388-01_WB_05_83-232.jpg)
![Western blot analysis of lysates from Rat brain using [KO Validated] CTIP1/BCL11A Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC1486_WB_02_84-339.jpg)
![Western blot analysis of various lysates using [KO Validated] Flotillin 1 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC76972-01_WB_01_83-630.jpg)
![Western blot analysis of lysates from wild type (WT) and FTO knockout (KO) 293T(KO) cells, using [KO Validated] FTO Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52115-01_KO-WB_01_82-277.jpg)
![Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from K-562, using 3 μg of [KO Validated] HDAC1 Rabbit mAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC70626-01_CHIP_01_83-308.jpg)
![Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH-1 Rabbit mAb at 1:16000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73051-01_WB_01_83-513.jpg)
![Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0256_KO-WB_01_81-037.jpg)