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![Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KO Validated] Smad3 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0490_KO-WB_01_82-639.jpg)
![Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1min.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0378_KO-WB_01_81-385.jpg)
![Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC0046_KO-WB_01_81-495.jpg)
![Western blot analysis of lysates from wild type (WT) and βIII-Tubulin knockout (KO) HeLa cells using [KO Validated] βIII-Tubulin Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC63789-01_WB_01_82-857.jpg)
