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![Western blot analysis of various lysates using [KO Validated] CDKN2A/p16INK4a Rabbit mAb at 1:15000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Negative control (NC): MCF7.|Exposure time: 10 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC3291-03BY_WB_01_83-182.jpg)