Monoclonal

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
Product Badges
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with YAP1 using HRP-conjugated Rabbit anti Myc-Tag mAb at 1:6000 dilution incubated overnight at 4℃.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of recombinant  goat IgG Fab protein using HRP-conjugated Rabbit anti-Goat IgG Fab mAb at 1:500-1:64000 dilution. Blot was incubated with the HRP conjugated antibody in the Blocking buffer (3% non-fat dry milk in TBST) at room temperature for 1 hour before detection using Enhanced chemiluminescence. |Lysates/proteins: Lane 1: molecular weight marker; Lanes 2-9: 100ng goat IgG Fab recombinant Protein per lane. |Detection: ECL Basic Kit. |Exposure time: 90s
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Goat
List Size:
100 uL
Western blot analysis of recombinant goat IgG Fc Protein using HRP-conjugated Rabbit anti-Goat IgG Fc mAb at 1:500-1:64000 dilution. Blot was incubated with HRP conjugated antibody in the Blocking buffer (3% nonfat dry milk in TBST) at room temperature for 1 hour before detection using Enhanced chemiluminescence. |Lysates/proteins: Lane 1: molecular weight marker; Lanes 2-9: 100ng goat IgG Fc recombinant Protein per lane. |Detection: ECL Basic Kit. |Exposure time: 15s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Goat
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit anti-Human IgA (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit anti-Human IgA (Fc) mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgA (Fc) while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Human IgE、Human IgD.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgD mAb measured by ELISA. 25 ng of Human IgD recombinant protein was coated in 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgD mAb in a 2 fold serial dilution from 1 μg/mL to 7.8 pg/mL, incubation was performed at room temperature for 1 hour. EC50 value ( 2.10×10^-2 μg/mL) were calculated using 4 parameter logistic regression.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgE (Fc) mAb in a 2 fold serial dilution from 2 μg/mL to 6.1×10^-5 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Anti-Human IgE (Fc) mAb has highly specific recognition of Human IgE while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Human IgM、Mouse IgG、Rat IgG.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of vlysates from Human plasma using HRP-conjugated Rabbit anti-Human IgG1 (Fc) mAb at 1:4000-1:32000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Rabbit Anti-Human IgG4 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgG4 mAb in a 2 fold serial dilution from 1 μg/mL to 7.8*10^-3 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit Rabbit Anti-Human IgG4 has highly specific recognition of Human IgG4 while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgM、Human IgE、Human IgD.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL
Dose response curve of HRP conjugated Human IgM mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate., blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Human IgM mAb in a 3 fold serial dilution from 0.5 μg/mL to 3.05×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Human IgM mAb has highly specific recognition of Human IgM while no or minimal cross reactivity to Human IgG1、Human IgG2、Human IgG3、Human IgG4、Mouse IgM、Rat IgM.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA
Conjugate:
HRP
List Size:
100 uL
Western blot analysis of recombinan Human IgG1 Kappa using HRP-conjugated Rabbit anti-Human Kappa Light Chain mAb at 1:500-1:32000 dilution incubated overnight at 4℃.|Lysates/proteins: 100ng μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 5s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
HRP
Species Reactivity:
Human
List Size:
100 uL