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Monoclonal

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit mAb at 1:10000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-663)

CATALOG NUMBER: 84-663

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb at 1:19000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-698)

CATALOG NUMBER: 84-698

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-RB-S795 Rabbit mAb at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100 ng/ml) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-RB-S795 Rabbit Recombinant Antibody (84-681)

CATALOG NUMBER: 84-681

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from L-929,using Phospho-RIP3-T231/S232 Rabbit mAb at 1:700 dilution. L-929 cells were treated with 20 ng/ml mouse TNF-a,100 nM BV-6 and 20 μM z-VAD for 5 or 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RIP3-T231/S232 Rabbit Recombinant Antibody (84-691)

CATALOG NUMBER: 84-691

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPA2-Ser33 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Camptothecin (1 μM) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-Ser33 Rabbit Recombinant Antibody (84-736)

CATALOG NUMBER: 84-736

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-T21 Rabbit Recombinant Antibody (84-600)

CATALOG NUMBER: 84-600

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-POLR2A-S2/S5 Rabbit mAb at 1:2000 dilution. HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 4s.

Phospho-Rpb1 CTD-S2/S5 Rabbit Recombinant Antibody (84-657)

CATALOG NUMBER: 84-657

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, CUT&Tag, ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of 293T, using Phospho-Rpb1 CTD-T4 Rabbit mAb at 1:2000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Rpb1 CTD-T4 Rabbit Recombinant Antibody (84-680)

CATALOG NUMBER: 84-680

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPS6-S235 Rabbit mAb at 1:3000 dilution. NIH/3T3 cells were treated with 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-RPS6-S235 Rabbit Recombinant Antibody (84-730)

CATALOG NUMBER: 84-730

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL