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Monoclonal

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Product Type
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Clonality
Host Species
Species Reactivity
Tested Applications
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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AKT1-S124 Rabbit Recombinant Antibody (84-561)

CATALOG NUMBER: 84-561

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb at 1:1000 dilution. MCF7 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AKT1-S129 Rabbit Recombinant Antibody (84-560)

CATALOG NUMBER: 84-560

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit mAb at 1:10000 dilution. RD cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447 Rabbit Recombinant Antibody (84-706)

CATALOG NUMBER: 84-706

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb at 1:1000 dilution. C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with PDGF (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-AKT1-T450 Rabbit Recombinant Antibody (84-559)

CATALOG NUMBER: 84-559

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-AKT1S1/PRAS40-T246 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. HeLa cells were treated with Insulin (50 nM) at 37℃ for 5 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 60 s.

Phospho-AKT1S1/PRAS40-T246 Rabbit Recombinant Antibody (84-739)

CATALOG NUMBER: 84-739

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with AKT2 using Phospho-AKT2-S474 Rabbit mAb at 1:1000 dilution. AKT2 transfected 293F cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-AKT2-S474(Akt2 Specific) Rabbit Recombinant Antibody (84-717)

CATALOG NUMBER: 84-717

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated with oligomycin (0. 5 μM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-AMPKα1-S496 Rabbit Recombinant Antibody (84-578)

CATALOG NUMBER: 84-578

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ASK1 using Phospho-ASK1-T845 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.293T cells transfected with ASK1 were treated by CIP (20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 5s.

Phospho-ASK1-T845 Rabbit Recombinant Antibody (84-771)

CATALOG NUMBER: 84-771

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
DB, ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from 293T cells using Phospho-ATM-S1981 Rabbit mAb at 1:8000 dilution incubated overnight at 4℃. 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ATM-S1981 Rabbit Recombinant Antibody (84-596)

CATALOG NUMBER: 84-596

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL