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Monoclonal

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
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Western blot analysis of lysates from 293T cells using Phospho-ATR-S428 Rabbit mAb at1:2000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.

Phospho-ATR-S428 Rabbit Recombinant Antibody (84-661)

CATALOG NUMBER: 84-661

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-ATR-S428 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with UV at room temperature for 15-30 minutes and λ-pp (1 mM) for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

Phospho-ATR-S428 Rabbit Recombinant Antibody (84-777)

CATALOG NUMBER: 84-777

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and A-549 cells transfected with Gas6 using Phospho-Axl-Y702 Rabbit mAb at 1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-Axl-Y702 Rabbit Recombinant Antibody (84-705)

CATALOG NUMBER: 84-705

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-B-Raf-T401 Rabbit Recombinant Antibody (84-601)

CATALOG NUMBER: 84-601

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-Bad-S112 Rabbit Recombinant Antibody (84-583)

CATALOG NUMBER: 84-583

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb at 1:7000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-Bad-S112 Rabbit Recombinant Antibody (84-669)

CATALOG NUMBER: 84-669

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-Beclin 1-S90 Rabbit mAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Phospho-Beclin 1-S90 Rabbit Recombinant Antibody (84-758)

CATALOG NUMBER: 84-758

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb at1:500 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. HeLa cells were treated with nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 300s.

Phospho-BRD4-S1083 Rabbit Recombinant Antibody (84-632)

CATALOG NUMBER: 84-632

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using at1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

Phospho-BRD4-T204 Rabbit Recombinant Antibody (84-633)

CATALOG NUMBER: 84-633

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL