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Monoclonal

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
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Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb at 1:1000 dilution. 293T cells were treated with Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-c-Myc-S62 Rabbit Recombinant Antibody (84-567)

CATALOG NUMBER: 84-567

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-c-Myc-T58 Rabbit mAb at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-c-Myc-T58 Rabbit Recombinant Antibody (84-568)

CATALOG NUMBER: 84-568

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-c-Myc-T58/S62 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-c-Myc-T58/S62 Rabbit Recombinant Antibody (84-566)

CATALOG NUMBER: 84-566

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from Mouse brain using Phospho-Camk2-T286 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. Mouse brain treated with CIP (1U/ul) at 37℃ for 1 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45 s.

Phospho-Camk2-T286 Rabbit Recombinant Antibody (84-683)

CATALOG NUMBER: 84-683

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3)-S23/S24 Rabbit mAb at dilution of 1:700 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Phospho-Cardiac Troponin I (TNNI3)-S23/S24 Rabbit Recombinant Antibody (84-688)

CATALOG NUMBER: 84-688

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-CDC37-S13 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-CDC37-S13 Rabbit Recombinant Antibody (84-618)

CATALOG NUMBER: 84-618

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (1 uM) at 37℃ for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 5s.

Phospho-Cdc6-S54 Rabbit Recombinant Antibody (84-626)

CATALOG NUMBER: 84-626

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Neuro-2a cells using Phospho-CDK1-T14 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. Neuro-2a cells were treated with Thymidine (2 nM) for 16 hours and Nocodazoleat (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Phospho-CDK1-T14 Rabbit Recombinant Antibody (84-728)

CATALOG NUMBER: 84-728

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL