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Monoclonal

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Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
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Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPA2-Ser33 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Camptothecin (1 μM) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-Ser33 Rabbit Recombinant Antibody (84-736)

CATALOG NUMBER: 84-736

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-RPA2-T21 Rabbit Recombinant Antibody (84-600)

CATALOG NUMBER: 84-600

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-POLR2A-S2/S5 Rabbit mAb at 1:2000 dilution. HeLa cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 4s.

Phospho-Rpb1 CTD-S2/S5 Rabbit Recombinant Antibody (84-657)

CATALOG NUMBER: 84-657

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, CUT&Tag, ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of 293T, using Phospho-Rpb1 CTD-T4 Rabbit mAb at 1:2000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Rpb1 CTD-T4 Rabbit Recombinant Antibody (84-680)

CATALOG NUMBER: 84-680

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-RPS6-S235 Rabbit mAb at 1:3000 dilution. NIH/3T3 cells were treated with 20% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-RPS6-S235 Rabbit Recombinant Antibody (84-730)

CATALOG NUMBER: 84-730

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:6000 dilution. NIH/3T3 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-645)

CATALOG NUMBER: 84-645

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S235/236 Rabbit mAb at 1:3000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with 10% FBS at 37℃ for 30 minutes. NIH/3T3 cells were treated with 10% FBS at 37℃ for 1.5 hours. PC-12 cells were treated with Anisomycin (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit Recombinant Antibody (84-646)

CATALOG NUMBER: 84-646

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution. HeLa and NIH/3T3 cell were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. PC-12 cells were treated with 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-647)

CATALOG NUMBER: 84-647

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein -S240/244 Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30 s.

Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit Recombinant Antibody (84-761)

CATALOG NUMBER: 84-761

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Phospho-SIRT1-S47 Rabbit Recombinant Antibody (84-556)

CATALOG NUMBER: 84-556

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL