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Monoclonal

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Product Type
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Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
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Western blot analysis of various lysates, using Phospho-PTEN-S380/T382/T383 Rabbit mAb at 1:2000 dilution. Hela,NIH/3T3,and PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-PTEN-S380/T382/T383 Rabbit Recombinant Antibody (84-655)

CATALOG NUMBER: 84-655

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb at 1:1000 dilution. A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit.|Exposure time: 3min.

Phospho-Rac1/Cdc42-S71 Rabbit Recombinant Antibody (84-587)

CATALOG NUMBER: 84-587

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb at 1:1000 dilution. Both 293T cells and C6 cells were treated with Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Raf1-S259 Rabbit Recombinant Antibody (84-585)

CATALOG NUMBER: 84-585

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Raf1-S621 Rabbit Recombinant Antibody (84-584)

CATALOG NUMBER: 84-584

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-RB-S780 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-550)

CATALOG NUMBER: 84-550

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit mAb at 1:10000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-663)

CATALOG NUMBER: 84-663

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb at 1:19000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RB-S780 Rabbit Recombinant Antibody (84-698)

CATALOG NUMBER: 84-698

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of various lysates, using Phospho-RB-S795 Rabbit mAb at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100 ng/ml) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

Phospho-RB-S795 Rabbit Recombinant Antibody (84-681)

CATALOG NUMBER: 84-681

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from L-929,using Phospho-RIP3-T231/S232 Rabbit mAb at 1:700 dilution. L-929 cells were treated with 20 ng/ml mouse TNF-a,100 nM BV-6 and 20 μM z-VAD for 5 or 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-RIP3-T231/S232 Rabbit Recombinant Antibody (84-691)

CATALOG NUMBER: 84-691

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL