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Monoclonal

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Clonality
Host Species
Species Reactivity
Tested Applications
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Western blot analysis of various lysates using Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with BMP4 (50 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467 Rabbit Recombinant Antibody (84-754)

CATALOG NUMBER: 84-754

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from293T cells using Phospho-Smad2-S245/S250/S255 Rabbit mAb at1:2000 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60 s.

Phospho-Smad2-S245/S250/S255 Rabbit Recombinant Antibody (84-650)

CATALOG NUMBER: 84-650

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-Smad2-S250 Rabbit mAb at 1:1000 dilution. 293T cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. C2C12 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Phospho-Smad2-S250 Rabbit Recombinant Antibody (84-582)

CATALOG NUMBER: 84-582

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb at 1:1000 dilution or Smad5 antibody. Hela cells were treated with ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Smad5-S463/S465 Rabbit Recombinant Antibody (84-590)

CATALOG NUMBER: 84-590

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S465 Rabbit mAb at 1:2000 dilution. NIH/3T3 cells were treated with BMP4(50ng/ml) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.

Phospho-Smad5-S465 Rabbit Recombinant Antibody (84-693)

CATALOG NUMBER: 84-693

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse, Rat
List Size:
100 uL

Phospho-SMAD9-Y92 Rabbit Recombinant Antibody (84-721)

CATALOG NUMBER: 84-721

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1s.

Phospho-SMC1-S957 Rabbit Recombinant Antibody (84-620)

CATALOG NUMBER: 84-620

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit mAb at 1:1000 dilution. C6 cells were treated with Pervanadate (0.5 mM) at 37℃ for 40 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-SRC Family-Y416 Rabbit Recombinant Antibody (84-668)

CATALOG NUMBER: 84-668

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3min.

Phospho-Src-Y419 Rabbit Recombinant Antibody (84-594)

CATALOG NUMBER: 84-594

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit mAb at 1:10000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Src-Y529 Rabbit Recombinant Antibody (84-675)

CATALOG NUMBER: 84-675

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL