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Affinity purification

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Host Species
Species Reactivity
Tested Applications
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Western blot analysis of lysates from wild type (WT) and 293F,293F-CCL25 cells transfected with CCL25 using CCL25 Rabbit mAb at 1:20000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 60s.

CCL25 Rabbit Recombinant Antibody (83-334)

CATALOG NUMBER: 83-334

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from Recombinant Human CCL3/MIP-1 alpha Protein using CCL3/MIP-alpha Rabbit mAb at 1:25000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng/5ng/1ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

CCL3/MIP-alpha Rabbit Recombinant Antibody (83-310)

CATALOG NUMBER: 83-310

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using CCL3/MIP-alpha Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45 s.

CCL3/MIP-alpha Rabbit Recombinant Antibody (83-311)

CATALOG NUMBER: 83-311

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using CCL4/MIP-1 beta Rabbit mAb at 1:11000 dilution incubated at room temperature for 1.5 hours. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight, LPS (1 μg/mL) and BFA (300 ng/mL) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 45s.

CCL4/MIP-1 beta Rabbit Recombinant Antibody (83-527)

CATALOG NUMBER: 83-527

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from THP-1 cells using CCL4/MIP-1 beta Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. THP-1 cells were treated with LPS (1 μg/ml), PMA/TPA (200 nM) and Brefeldin A (300 ng/ml) for 8 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 10s.

CCL4/MIP-1 beta Rabbit Recombinant Antibody (83-544)

CATALOG NUMBER: 83-544

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using CCR2/CKR2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.

CCR2/CKR2 Rabbit Recombinant Antibody (82-772)

CATALOG NUMBER: 82-772

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using CCR7 Rabbit mAb at 1：1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

CCR7 Rabbit Recombinant Antibody (81-012)

CATALOG NUMBER: 81-012

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using CCR8 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

CCR8 Rabbit Recombinant Antibody (83-872)

CATALOG NUMBER: 83-872

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of various lysates using CCR9 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

CCR9 Rabbit Recombinant Antibody (84-462)

CATALOG NUMBER: 84-462

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb at 1:3000 dilution incubated overnight at 4°C.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. |Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|Exposure time: 60 s.

CD10 Rabbit Recombinant Antibody (83-138)

CATALOG NUMBER: 83-138

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL