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Apoptosis

Categories

Product Type
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
Product Badges
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3s.

Phospho-HSP27/HSPB1-S78 Rabbit Recombinant Antibody (84-598)

CATALOG NUMBER: 84-598

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3s.

Phospho-HSP27/HSPB1-S82 Rabbit Recombinant Antibody (84-597)

CATALOG NUMBER: 84-597

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from HuH-7 cells using Phospho-JAK2-Y1007/1008 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. HuH-7 cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 5s.

Phospho-JAK2-Y1007/1008 Rabbit Recombinant Antibody (84-775)

CATALOG NUMBER: 84-775

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with Etoposide (25 uM) at 37℃ for 5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 60s.

Phospho-KAP1/TRIM28-S824 Rabbit Recombinant Antibody (84-686)

CATALOG NUMBER: 84-686

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-MST1-T183+MST2-T180 Rabbit mAb at 1:40000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 20s.

Phospho-MST1-T183+MST2-T180 Rabbit Recombinant Antibody (84-769)

CATALOG NUMBER: 84-769

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-NF-κB p105-S932 Rabbit mAb at 1:9000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and C6 cells were treated with TNF-α (20 ng/mL) and CA (100 nM) at 37℃ for 10 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-NF-κB p105-S932 Rabbit Recombinant Antibody (84-658)

CATALOG NUMBER: 84-658

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866 Rabbit mAb at 1:500 dilution. NIH/3T3 cells were treated with TNF-α (20 ng/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-NF-κB2-S866 Rabbit Recombinant Antibody (84-666)

CATALOG NUMBER: 84-666

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL