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![Western blot analysis of various lysates using [KO Validated] IRF3 Rabbit mAb at 1:11000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC73216-01_WB_01_83-501.jpg)
![Western blot analysis of various lysates using [KO Validated] KAT1/HAT1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10 s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC1002_WB_01_83-919.jpg)
![Western blot analysis of lysates from wild type (WT) and Lactate Dehydrogenase B/LDH-B knockout (KO) 293T cells using [KO Validated] Lactate Dehydrogenase B/LDH-B Rabbit mAb at 1:12000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC71823-01_WB_01_83-531.jpg)
![Western blot analysis of various lysates using [KO Validated] Lamin A/C Mouse mAb at 1:10000 dilution incubated overnight at 4℃.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 45s.](https://www.prosciantibodies.com/wp-content/uploads/products/AMC50041-01_WB_01_83-344.jpg)
![Western blot analysis of lysates from wild type(WT) and LAMP1 knockout (KO) HeLa(KO) cells, using [KO Validated] LAMP1/CD107a Rabbit mAb at 1:10000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC52155-01_KO-WB_01_82-213.jpg)
![Western blot analysis of lysates from A549 cells using [KO Validated] LAMP2 Rabbit mAb at 1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time:10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC54767-01_WB_01_83-132.jpg)
![Western blot analysis of lysates from MCF7 cells, using [KO Validated] LDHA Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC2669_WB_01_81-082.jpg)
![Western blot analysis of various lysates, using [KO Validated] MSH6 Rabbit mAb at 1:3000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC57727-01_WB_01_83-129.jpg)
![Western blot analysis of lysates from wild type (WT) and MTusing [KO Validated] MTA2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC1056_KO-WB_01_83-964.jpg)
![Western blot analysis of lysates from wild type (WT) and N-terminal alpha-synuclein knockout (KO) HIPP Neurons cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] at 1:5000 dilution .|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/products/ARC5166-01_WB_01_83-469.jpg)