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Monoclonal

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Product Type
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Clonality
Host Species
Species Reactivity
Tested Applications
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Western blot analysis of lysates from A-431 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb at 1:19000 dilution incubated overnight at 4℃. A-431 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90 s.

Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit Recombinant Antibody (84-765)

CATALOG NUMBER: 84-765

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-CDK1-Y15 Rabbit mAb at 1:2000 dilution. HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3 s.

Phospho-CDK1-Y15 Rabbit Recombinant Antibody (84-656)

CATALOG NUMBER: 84-656

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates using Phospho-Cdk1/2-T14 Rabbit mAb at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Cdk1/2-T14 Rabbit Recombinant Antibody (84-577)

CATALOG NUMBER: 84-577

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of various lysates using Phospho-CDK1/2/3-T14 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Both NIH/3T3 cells and C6 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 30 s.

Phospho-CDK1/2/3-T14 Rabbit Recombinant Antibody (84-616)

CATALOG NUMBER: 84-616

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells, using Phospho-CDK2-T160 Rabbit mAb at 1:5000 dilution. HeLa cells were treated with nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.

Phospho-CDK2-T160 Rabbit Recombinant Antibody (84-665)

CATALOG NUMBER: 84-665

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF-P, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from HeLa cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with Nocodazole (20ng/ml).|Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-CDK2-T160 Rabbit Recombinant Antibody (84-734)

CATALOG NUMBER: 84-734

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Rat
List Size:
100 uL
Western blot analysis of lysates from NIH/3T3 cells using Phospho-CDK2-Y15 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3 s.

Phospho-CDK2-Y15 Rabbit Recombinant Antibody (84-581)

CATALOG NUMBER: 84-581

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from MCF7 cells using Phospho-CEBPB-T235 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. MCF7 cells were treated with U0126 (20 μM) for 48 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90s.

Phospho-CEBPB-T235 Rabbit Recombinant Antibody (84-772)

CATALOG NUMBER: 84-772

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IHC-P, WB
Conjugate:
Unconjugated
Species Reactivity:
Human, Mouse, Rat
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-Chk1 (S280) Rabbit mAb at 1:500 dilution. 293T cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Phospho-Chk1-S280 Rabbit Recombinant Antibody (84-704)

CATALOG NUMBER: 84-704

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit mAb at 1:2000 dilution. 293T cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 20s.

Phospho-Chk1-S280 Rabbit Recombinant Antibody (84-715)

CATALOG NUMBER: 84-715

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL