Monoclonal

Product Type
Research Area
Clonality
Host Species
Species Reactivity
Tested Applications
Modifications
Conjugate
Product Badges
Western blot analysis of lysates from wild type (WT)293F cells, 293F cells transfected with Snail-His-tag and 293F cells transfected with CYP1A2-His-tag using Rabbit anti His-tag mAb at 1:11000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 10 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IF/ICC, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Human IgG4 (Fc) using Rabbit anti Human IgG4 (Fc)  mAb at  dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 3s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with mCherry using Rabbit anti mCherry-Tag mAb at 1:20000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 45s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, IP, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) 293F cells, 293F cells transfected with FOXA1-V5-Tag and 293F cells transfected with GFP-V5-Tag using Rabbit anti V5-Tag mAb at 1:11000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 10 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ChIP, ELISA, IF/ICC, WB
Conjugate:
Unconjugated
Species Reactivity:
Species independent
List Size:
100 uL
Western blot analysis of various lysates using Rabbit Anti-Chicken IgY (H+L) at 1:10000 dilution.| Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.| Lysates / proteins: 5 ng - 10 ng per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 90s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
List Size:
100 uL
Western blot analysis of lysates from Chicken IgY Fab, using Rabbit Anti-Chicken IgY Fab at1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.

CATALOG NUMBER: 84-778

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
List Size:
100 uL
Western blot analysis of recombinant Goat IgG (Fc) fragment and Goat IgG (Fab) fragment(Negative control) , using Rabbit Anti-Goat IgG (Fc) fragment Rabbit mAb at  1:5000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 180s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Goat
List Size:
100 uL
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Human IgG2 using Rabbit Anti-Human IgG2 (Fc) mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 20 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit|.Exposure time: 30s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Conjugate:
Unconjugated
Species Reactivity:
Human
List Size:
100 uL
Western blot analysis of various lysates, using Rabbit Anti-Mouse IgG2a at  1:160000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

CATALOG NUMBER: 82-385

Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL
Western blot analysis of lysates from Purified Mouse IgG3κ Isotype Ctrl Antibody using Rabbit anti-Mouse IgG3 (Fc) mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody:  HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 10 ng per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90 s.
Clonality:
Monoclonal
Host Species:
Rabbit
Tested Applications:
ELISA, FC, WB
Conjugate:
Unconjugated
Species Reactivity:
Mouse
List Size:
100 uL